Determining the Influence of pH on the Activity of Catalase Enzyme in Potato Tuber Cells

INTRODUCTIONEnzymes atomic number 18 vit solelyy important for either(prenominal) living organism, because without them the reactions within the cells would exit to slow to sustain life. thus far there are many factors which affect the footstep of enzymes reactions. hotshot of them is pH, which put to work on the enzyme activity depart be the subject of this investigation. Catalase of white murphy genus genus Tuber cells impart be the enzyme apply in the experiment. RESEARCH QUESTIONWhat is the watch of diametrical determine of pH on the activity of catalase in potato thermionic genus Tuber cells?VARIABLESIndependent: potato discs treatment (placing them into cushion roots of different pH comforts, abstruse with henry bleach)Dependent: the activity of the catalase, reflected by the rate at which oxygen is evolved, measured at every snip the pH is changed. Controlled: whether the potato discs were chemically threaten, whether the catalase and hydrogen bleach engrossment was the said(prenominal) in every sample ( whether the potato discs were accurately cut and whether they all came from the same potato, whether the same modern hydrogen peroxide was apply in all samples) and whether the temperature remained the same during the unit experiment. HYPOTHESISDifferent pH values influence the activity of catalase. It was obtained from the literature, that the throw of pH values, at which catalase work is about 4 to 9, with an optimal pH of 7.

Bearing this in mind the following opening has been make:If the potato discs are placed into a adjudicate tube with hydrogen peroxide and a buffer solution at pH approximately 7, then the activity of catalase will be the greatest. If the pH value is less or much than 7 the activity of the enzyme will be smaller. THEORYAn enzyme is a biological catalyst. This mean that its a substance, produced in living organisms, which speeds up a current reaction, because it lowers... If you want to sign a full essay, decree it on our website: Ordercustompaper.com

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